USE OF CELL SELECTION IN OBTAINING STRESS RESISTANT TOBACCO (NICOTIANA TABACUM L.) TO DELIPIDATED SALINITY AND ANTHROPOGENIC POLLUTION
DOI:
https://doi.org/10.31812/ecobulletinkrd.8340Keywords:
salinity, cell selection, resistance, Nicotiana tabacum L., stress tolerance, K+ and Na+ ions, regenerant, ptotein, linesAbstract
Soil salinity is one of the environmental factors that lead to a significant reduction in plant yields. Due to the overall deterioration of the global environmental picture and the widespread use of artificial irrigation, there is a growing need to breed salt-resistant plant species. The use of plant cell culture also makes it possible to study stress resistance. The advantage of in vitro testing of plant resistance to abiotic factors is the rapid assessment of polygenic resistance. The use of the cell selection method with the application of lethal doses of IPM is a guaranteed method of isolating cell cultures that are characterised by active vital activity under the influence of such a stress load. The tobacco cell lines resistant to barium cations (Ba-CCL) were obtained. The frequency of isolation of resistant variants in any genotype corresponded to the order of 10-6. In cell breeding, resistance is assessed by the relative increase in callus biomass (∆m), which indicates the degree of vital activity (stress damage). The applied concentration of Ba2+ cations was lethal for wild-type cell cultures. The obtained resistant cultures were considered to be resistant tobacco cell lines, which was proved by further results. They were characterised by stable proliferation under the influence of stressor ions and retained this feature in cell generations after prolonged cultivation without selective pressure.
Downloads
References
Abbas, A., Mansha, S., Wahud, H., Hayyat, U., Zhang, Y.-J., Alwutayd, K. (2024). NaCl stress, tissue specific Na+ and K+ uptake and their effect on growth and physiology of Helianthus annus L. Scientia Horticulture, 326, 112454. https://doi.org/10.1016/j.scienta.2023.112454
Ahmed, S., Ahmed, S., Roy, S.K., Woo, S.H., Sonawane, D., Shohael, A.M. (2022). Effect of salinity on the morphological, physiological and biochemical properties of lettuce (Lactuca sativa L.) in Bangladesh. Open Agriculture, 4, 361–373. https://doi.org/10.1515/opag-2019-0033
Andreeva, T.V., Maluchenko, N.V., Sivkuna, A.L., Chertkov, O.V., Valieva, M.E., Kotova, E.Y., Kirpichnikova, M.P., Studitsky, V.M., Feofanov, A.V. (2022). Na+ and K+ ions differently affect nucleosome structure and interactions with proteins. Microscopy and Microanalysis, 28(1), 243–253. https://doi.org/10.1017/S14319276211013751
Bangajavilli, S., Selvaraj, K., Esakkimal, B. (2021). Retrieval of barium affected Phaseolus mungo L. by Ulva lactuca. International Journal of Environmental Science and Natural Resources, 6(4), 59–63. https://doi.org/10.19080/IJESNR.2018.08.555729
Bapat, V.A., Kavi Kishor, P.B., Jalaja, N., Jain, S.M., Penna, S. (2023). Plant cell cultures: biofactories for the production of bioactive compounds. Agronomy, 13(3), 857. https://doi.org/10.3390/agronomy13030858
Bull, T., Michelmore, R. (2022). Molecular determinants of in vitro plant regeneration: prospects for enhanced manipulation of lettuce (Lactuca sativa L.). Frontiers in Plant Science, 13, 1–32. https://doi.org/10.3389/fpls.2022.888425
Brini, F., Saibi, W. (2023). Role of proline in regulating physiological and molecular aspects of plants under abiotic stress. In: Chapter 14, 39–47. https://doi.org/10.1016/B978-0-323-98332-7.00007-X
Colin, I., Ruhnow, F., Zhu, L.-K., Zhao, Y., Person, S. (2023). The cell biology of primary cell walls during salt stress. The Plant Cell, 35(1), 201–207. https://doi.org/10.1093/plcell/koa292
Imran, Q.M., Falak, N., Hussan, A., Man, B.-G., Yun, B.-W. (2021). Abiotic stress biotechnological tools in stress response. Agronomy, 11(8), 1579. https://doi.org/10.3390/agronomy11081579
Jiang, L., Pang, L., Yang, L., Li, W., Duan, L., Zhang, G., Luo, Y. (2021). Engineering endogenous L-proline biosynthetic pathway to boost trans-4-hydroxy-L-proline production in Escherichia coli. Journal of Biotechnology, 4(1), 70–79. https://doi.org/10.1016/j.jbiotech.2021.01.015
Kamiloglu, S., Tomas, M., Ozkan, G., Ozbal, T., Capanoglu, E. (2024). In vitro digestibility of plant proteins: strategies for improvement and health implications. Current Opinion in Food Science, 57, 101148. https://doi.org/10.1016/j.cofs.2024.101148
Kobata, T. (2024). Abiotic factors affect plant growth. In: Responses of Plants to Soil Flooding, 3–17. https://doi.org/10.1007/978-981-99-9112-9_1
Li, M., Zou, L., Zhang, L., Ren, G., Liu, Y., Zhao, X. (2024). Plant-based proteins: advances in their sources, digestive profiles in vitro and potential health benefits. Critical Reviews in Food Science and Nutrition, 1–21. https://doi.org/10.1080/10408398.2024.2315488
Luo, D., Song, F., Lu, M., Shi, Y., Ma, Q. (2023). Salt stress-induced ion transport contributes to K+/Na+ homeostasis in roots of Ping’on hybrid hazelnut. Forests, 14(8), 1851. https://doi.org/10.3390/f14081851
Lohani, N., Singh, M., Bhalla, P. (2022). Biological parts for engineering abiotic stress tolerance in plants. BioDesign Research, 2022, 9819314. https://doi.org/10.34133/2022/9819314
Meca, A., Schwartz, S. (2024). Cultural stress theory: an overview. Cultural Diversity and Ethnic Minority Psychology, 4, 603–612. https://doi.org/10.1037/cdp0000704
De Paolis, A., Frugis, G., Giannino, D., Iannelli, M.A., Mele, G., Rugini, E., Silvestri, C., Sparvoli, F., Testone, G., Mauro, M.L., Nicolodi, C., Caretto, S. (2019). Plant cellular biotechnology: following Mariotti’s steps. Plants, 8(1), 18. https://doi.org/10.3390/plants8010018
Moukhtari, A.R., Cabassa-Hourton, C., Farissi, M., Savouré, A. (2020). How does proline treatment promote salt stress tolerance during crop plant development? Frontiers in Plant Science, 11, 1127. https://doi.org/10.3389/fpls.2020.01127
Renzetti, M., Funk, D., Trovato, M. (2025). Proline and ROS: a unified mechanism in plant development and stress response. Plants, 14(1), 2. https://doi.org/10.3390/plants14010002
Sergeeva, L.E., Bronnikova, L.I. (2019). Cadmium ions in cell selection for obtaining wheat cell forms tolerant to water stress. Bulletin of Cherkasy University. Series: Biology, 2, 74–80. https://doi.org/10.31651/2076-5835-2018-1-2019-2-74-80
Sergeeva, L.E., Bronnikova, L.I. (2020). Cell selection with barium ions for obtaining genetically modified tobacco. Bulletin of Cherkasy University. Series: Biology, 1, 71–78. https://doi.org/10.31651/1076-5835-2018-1-2020-1-71-78
Slemi, N., Kouki, R., Ammar, M.H., Ferrer, R., Pérez-Clemente, R. (2021). Barium effect on germination, plant growth and antioxidant enzymes in Cucumis sativus L. Food Science & Nutrition, 9(4), 2086–2094. https://doi.org/10.1002/fsn3.2177
Yuan, T.T., Xiang, Z.X., Li, W., Gao, X., Lu, Y.T. (2021). Osmotic stress represses root growth by modulating the transcriptional regulation of PIN-FORMED3. New Phytologist, 232(1), 1661–1673. https://doi.org/10.1111/nph.17687
